It depends where you hold you database, if you are not using raw disk(s) for DB then you can use incremental updates, but only for Windows, and don't loose data between initial transfer and still running DB, before final update you must shutdown server and this will be your down time for server. However this is the ideal scenario, most often the server will not run smoothly on destination VM because of hardware (including network!) changes. You should search forum for best practices, there are many posts about conversion of MS SQL servers. HTH
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